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Laboratory of Stem Cell Biology

Major Research Interest of the Lab

  • Combinatorial approaches of stem cells therapy for myocardial infarction

  • Cell-type specific miRNAs involved in cardiac fibrosis associated to natural ageing process

  • Mesenchymal stem cell-based approaches for treatment of diabetic autoimmunity

Ongoing Projects

  • Transnational Research Projects on Cardiovascular Diseases, 2016-2019, EXPERT (Eur 200,000)

  • Improve institutional competitiveness in the field of type 1 diabetes by developing an innovative concept of immunotherapy based on mesenchymal stromal cells, 2016-2020, DIABETER (Eur 2,000,000)

  • Interference with hypoxia-signaling pathways MSC prior to transplantation as a strategy to enhance myocardial recovery post infarction, 2018-2020, OXI-SCENARIO (Eur 60,000)

Current Collaborations

Nadir Askenasy, MD, PhD (Frankel Laboratory, Centre for Stem Cell Research, Schneider Children’s Medical Centre of Israel)

Mircea Ivan, MD, PhD, (Associate Professor of Microbiology & Immunology at Indiana University School of Medicine, USA)

Elisa Liehn, MD, PhD (RWTH Aachen University, Germany)

Expertise

  • Cell culture: derivation of cell lines (human  and mouse Mesenchymal Stem Cells; huma Endothelial Progenitor Cells, adult and neonatal murine cardiomyocytes, adult cardiac fibroblasts, embryonic stem cells, etc.), in vitro cell culture in hypoxic conditions (H35 hypoxystation, Don Whitley Scientific); isolated heart perfusion using Langendorff system; cell-cell interactions; xCELLigence analysis; metabolic analysis (Seahorse analyzer), biocompatibility assays, 3D culture systems (aggregates, co-cultures, hanging-drops).

  • Microsurgery: mouse model of myocardial infarction or ischemia-reperfusion injury (by permanent or transient ligation of the left coronary artery) and mouse model of hind limb ischemia (by femoral vein ligation and excision); Cell Transplant.

  • Imaging: microscopy (Leica DMi8 inverted microscope and TCS-SP5 confocal microscope), ecocardiography (VEVO 2100), in vivo imaging (IVIS Spectrum, Perkin Elmer), Flow Cytometry (CytoFlex and MoFlo Astrios, Beckman Coulter).

  • Molecular biology: cloning, transfection, qRT-PCR, PCR.

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